beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48328

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM617

Format

IHC-Prediluted
View all beta 2-Microglobulin Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769)

Reactivity Notes

Non-Human primates.

Localization

Cell Surface and Extracellular (Secreted)

Marker

Renal Failure & Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma
beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted Flow Cytometry: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] -

Flow Cytometry: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] -

Flow Cytometric Analysis of PFA fixed HeLa cells using beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted followed by Goat anti-mouse IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 Is Control (Red).
beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted Western Blot: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] -

Western Blot: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] -

Western Blot Analysis of THP-1 and Raji Cell lysate using beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted.
beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] -

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted [NBP2-48328] -

Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted; followed by goat anti-mouse IgG-CF488 (Green).

Applications for beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for beta 2-Microglobulin Antibody (SPM617) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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