beta-Arrestin 2 Antibody

Novus Biologicals | Catalog # NB300-587

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all beta-Arrestin 2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 19776273). Rat reactivity reported in scientific literature (PMID: 19200235).

Specificity

Detects recombinant rat beta-arrestin and beta-arrestin 2. This does not detect visual or cone arrestin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Arrestin 2 Antibody

Western Blot: beta-Arrestin 2 Antibody [NB300-587]

Western Blot: beta-Arrestin 2 Antibody [NB300-587]

Western Blot: beta-Arrestin 2 Antibody [NB300-587] - Analysis of rat retina.

Applications for beta-Arrestin 2 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

1:2000
Application Notes
WB: Detects an approx. 49 kDa and ~47 kDa proteins representing pan Arrestin in rat and bovine retina as well as rat brain samples.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Arrestin 2

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin.

Alternate Names

ARB2, ARR2, ARRB2, betaArrestin 2

Entrez Gene IDs

409 (Human); 25388 (Rat); 281638 (Bovine)

Gene Symbol

ARRB2

UniProt

P32121

Additional beta-Arrestin 2 Products

Product Documents for beta-Arrestin 2 Antibody

Certificate of Analysis

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Product Specific Notices for beta-Arrestin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-Arrestin 2 Antibody

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail