beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

Novus Biologicals | Catalog # H00002720-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6E7

Format

Azide and BSA Free
View all beta-Galactosidase-1/GLB1 Primary Antibodies »

Product Specifications

Immunogen

GLB1 (NP_000395, 578 a.a. ~ 677 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

Specificity

GLB1 - galactosidase, beta 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - Analysis of GLB1 expression in transfected 293T cell line by GLB1 monoclonal antibody (M01), clone 6E7.Lane 1: GLB1 transfected lysate(76.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - GLB1 monoclonal antibody (M01), clone 6E7 Analysis of GLB1 expression in HeLa.
Immunoprecipitation: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

Immunoprecipitation: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

Immunoprecipitation: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - Analysis of GLB1 transfected lysate using anti-GLB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLB1 MaxPab rabbit polyclonal antibody.
ELISA: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

ELISA: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01]

ELISA: beta-Galactosidase-1/GLB1 Antibody (6E7) [H00002720-M01] - Detection limit for recombinant GST tagged GLB1 is 0.03 ng/ml as a capture antibody.

Applications for beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-Galactosidase-1/GLB1

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Alternate Names

betaGalactosidase1, EBP, ELNR1, GLB1, Lactase

Entrez Gene IDs

2720 (Human)

Gene Symbol

GLB1

OMIM

230500 (Human)

UniProt

NP_000395 (Human)

Additional beta-Galactosidase-1/GLB1 Products

Product Documents for beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta-Galactosidase-1/GLB1 Antibody (6E7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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