beta-Glucuronidase/GUSB Antibody (4U0E2)

Novus Biologicals | Catalog # NBP3-15711

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4U0E2 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta-Glucuronidase/GUSB (NP_000172.2). MARGSAVAWAALGPLLWGCALGLQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Glucuronidase/GUSB Antibody (4U0E2)

Western Blot: beta-Glucuronidase/GUSB Antibody (4U0E2) [NBP3-15711]

Western Blot: beta-Glucuronidase/GUSB Antibody (4U0E2) [NBP3-15711]

Western Blot: beta-Glucuronidase/GUSB Antibody (4U0E2) [NBP3-15711] - Western blot analysis of extracts of various cell lines, using beta glucuronidase (beta-Glucuronidase/GUSB) antibody (NBP3-15711) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for beta-Glucuronidase/GUSB Antibody (4U0E2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Glucuronidase/GUSB

The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM]

Alternate Names

Beta-G1, MPS7

Gene Symbol

GUSB

Additional beta-Glucuronidase/GUSB Products

Product Documents for beta-Glucuronidase/GUSB Antibody (4U0E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for beta-Glucuronidase/GUSB Antibody (4U0E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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