Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

Name: Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-59955
Price: 749 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-59955

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Product Documents

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and milk (50 - 100 uL)

Sensitivity

0.129 ug/mL

Assay Range

0.156 ug/mL - 10 ug/mL

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Bovine

Specificity

This assay has high sensitivity and excellent specificity for detection of bovine LGB. No significant cross-reactivity or interference between bovine LGB and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Recovery for Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

Recovery

89 - 100%

Scientific Data Images for Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

ELISA: Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) [NBP2-59955]

ELISA: Bovine Beta Lactoglobulin ELISA Kit [NBP2-59955] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) [NBP2-59955] -

Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) [NBP2-59955] - Standard Curve Reference

Kit Contents for Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

Adhesive Strip (For 96 wells)
Assay plate
Biotin-conjugate (100 x concentrate)
Biotin-conjugate Diluent
HRP-avidin (100 x concentrate)
HRP-avidin Diluent
Instruction manual
Sample Diluent
Standard (Freeze dried)
Stop Solution
TMB Substrate
Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Beta Lactoglobulin

This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for LGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated LGB. A competitive inhibition reaction is launched between LGB (Standards or samples) and biotin-conjugated LGB with the pre-coated LGB antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of LGB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional Beta Lactoglobulin Products

Product Documents for Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

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Download Manual - Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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