BI dihydrochloride

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BI dihydrochloride | Light-up Aptamers
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Description: DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers

Chemical Name: (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride

Purity: ≥98%

Product Details

Biological Activity

BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (KD values are 51 and 305 nM, respectively).

Optical Data for BI dihydrochloride

Emission Color Green
λabs 470 nm
λem 505 nm
Extinction Coefficient (ε) 33600 M-1cm-1
Quantum Yield (φ) 0.669
Cell Permeable Yes
Application DFHBI Derivative for Imaging of RNA in Living Cells

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Technical Data

Soluble to 100 mM in water and to 50 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from Lucerna

Product Datasheets

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