Bim Antibody

Novus Biologicals | Catalog # NB100-1440

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Bim Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-FNAYYARRLEK corresponding to C-Terminus according to NP_619528.1, NP_619529.1, NP_996886.1.

Localization

Intracytoplasmic membranes

Specificity

This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Bim Antibody

Western Blot: Bim Antibody [NB100-1440]

Western Blot: Bim Antibody [NB100-1440]

Western Blot: Bim Antibody [NB100-1440] - (0.5ug/ml) of K562lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for Bim Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 23 kDa band observed in K562 lysates (predicted MW of 18.5 kDa band according to NP_619528.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bim

Bim belongs to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw.

Alternate Names

BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL

Entrez Gene IDs

10018 (Human)

Gene Symbol

BCL2L11

UniProt

NP_619528 (Human)

Additional Bim Products

Product Documents for Bim Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bim Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Bim Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A: Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A: Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

Showing  1 - 2 of 2 FAQs Showing All
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