Biotin Antibody (1D4-C5) - BSA Free

Novus Biologicals | Catalog # NBP3-23510

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D4-C5

Format

BSA Free
View all Biotin Primary Antibodies »

Product Specifications

Immunogen

Biotinylated Limulus hemocyanin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Biotin Antibody (1D4-C5) - BSA Free

ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] -

ELISA: Biotin Antibody (1D4-C5) [NBP3-23510] - ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 ug/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in dose dependent manner, but with different dynamics, when biotinylated antibody was detected by anti-biotin mAb/goat anti mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in dose dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).

Applications for Biotin Antibody (1D4-C5) - BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody (1D4-C5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Biotin Antibody (1D4-C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Biotin Antibody (1D4-C5) - BSA Free

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  • Q: How large is Biotin label?

    A: Biotin is 244 Daltons (244.31 g/mol). This small size means the biological activity of a labled protein will most likely be unaffected by the addition of biotin.

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