Biotin Antibody (7H10R8)

Novus Biologicals | Catalog # NBP3-16062

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG1 Clone # 7H10R8 expressed in HEK293
View all Biotin Primary Antibodies »

Product Specifications

Immunogen

Biotin

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG1

Scientific Data Images for Biotin Antibody (7H10R8)

Biotin Antibody (7H10R8)

Western Blot: Biotin Antibody (7H10R8) [NBP3-16062] -

Western Blot: Biotin Antibody (7H10R8) [NBP3-16062] - Western blot analysis of various lysates, using Biotin Rabbit mAb at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for Biotin Antibody (7H10R8)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody (7H10R8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Biotin Antibody (7H10R8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Biotin Antibody (7H10R8)

There are currently no reviews for this product. Be the first to review Biotin Antibody (7H10R8) and earn rewards!

Have you used Biotin Antibody (7H10R8)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Biotin Antibody (7H10R8)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: How large is Biotin label?

    A: Biotin is 244 Daltons (244.31 g/mol). This small size means the biological activity of a labled protein will most likely be unaffected by the addition of biotin.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies