Biotin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15606

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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Biotin Primary Antibodies »

Product Specifications

Immunogen

Biotin

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Biotin Antibody - Azide and BSA Free

Biotin Antibody - Azide and BSA Free

Western Blot: Biotin Antibody - Azide and BSA Free [Biotin] -

Western Blot: Biotin Antibody - Azide and BSA Free [Biotin] - Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 100 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Applications for Biotin Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Biotin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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FAQs for Biotin Antibody - Azide and BSA Free

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  • Q: How large is Biotin label?

    A: Biotin is 244 Daltons (244.31 g/mol). This small size means the biological activity of a labled protein will most likely be unaffected by the addition of biotin.

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