Biotin-NAD+

Name: Biotin-NAD+
Brand: Tocris Bioscience
SKU: 6573
Price: 463 USD
Availability: InStock

Tocris Bioscience | Catalog # 6573

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Citations (10)

Product Documents

Biotinylated-NAD+; substrate for ADP-ribosylation

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Product Description
Product Specifications
Solubility
Calculators
Background References
Product Documents
Specific Notices

Key Product Details

Description

Biotinylated-NAD+; substrate for ADP-ribosylation

View all Biochemicals and Molecular Biology Compounds »

Product Description

Biotin-NAD+ is a provides a convenient non-isotopic alternative to radiolabeled NAD⁺ for determination of IC₅₀ values for candidate PARP inhibitors and studies requiring this substrate. Biotinylated-NAD⁺ allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system. Acts as a substrate for ADP-ribosylation. Can be used to label and purify biotinyl-ADP ribosylated proteins.

This product is a replacement for R&D Systems product 4670-500-01.

131 μg is supplied as 500 μl of a 0.25 mM solution in water.

Product Specifications for Biotin-NAD+

Molecular Weight

1045.95

Formula

C₃₉H₅₇N₁₁O₁₇P₂S

Storage

Store at -80°C

Chemical Name

β-Nicotinamide-N⁶-[2-[[6-[biotinyl]amino]hexyl]amino]-2-oxoethyl]adenine dinucleotide

CAS Number

146385-37-7

InChI Key

MIIADCCJYSONEI-RNWYXDGLSA-N

SMILES

[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCCNC(CNC3=C(N=CN4[C@@H]5O[C@@H]([C@H]([C@H]5O)O)COP(O)(OP(OC[C@H]6O[C@@H]([N+]7=CC=CC(C(N)=O)=C7)[C@H](O)[C@@H]6O)([O-])=O)=O)C4=NC=N3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Soluble in water (supplied pre-dissolved at a concentration of 0.25mM)

Calculators

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Mass

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.

Yang Antitumor activity of a pyrrole-imidazole polyamide. Proc.Natl.Acad.Sci.USA 2013 PMID: 23319609
Yang Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9 Mol. Cell 2017 PMID: 28525742
Schuster The Hsp90 machinery facilitates the transport of diphtheria toxin into human cells. Sci.Rep. 2017 PMID: 28377614
Zhang Purification of a nitric oxide-stimulated ADP-ribosylated protein using biotinylated beta-nicotinamide adenine dinucleotide. Biochemistry 1993 PMID: 8443164

Product Documents for Biotin-NAD+

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Biotin-NAD+

For research use only

Citations for Biotin-NAD+

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