BLMH/Bleomycin Hydrolase Antibody (4A2) - Azide and BSA Free

Novus Biologicals | Catalog # H00000642-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A2

Format

Azide and BSA Free
View all BLMH/Bleomycin Hydrolase Primary Antibodies »

Product Specifications

Immunogen

BLMH (NP_000377, 356 a.a. ~ 454 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDRKHVPEEVLAVLEQEPIILPAWDPMGALA

Specificity

BLMH - bleomycin hydrolase (4A2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BLMH/Bleomycin Hydrolase Antibody (4A2) - Azide and BSA Free

Western Blot: BLMH/Bleomycin Hydrolase Antibody (4A2) [H00000642-M02]

Western Blot: BLMH/Bleomycin Hydrolase Antibody (4A2) [H00000642-M02]

Western Blot: BLMH/Bleomycin Hydrolase Antibody (4A2) [H00000642-M02] - BLMH monoclonal antibody (M02), clone 4A2. Analysis of BLMH expression in K-562.
Sandwich ELISA: BLMH/Bleomycin Hydrolase Antibody (4A2) [H00000642-M02] - Detection limit for recombinant GST tagged BLMH is approximately 0.3ng/ml as a capture antibody.

Applications for BLMH/Bleomycin Hydrolase Antibody (4A2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BLMH/Bleomycin Hydrolase

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]

Alternate Names

BMH

Entrez Gene IDs

642 (Human)

Gene Symbol

BLMH

UniProt

NP_000377 (Human)

Additional BLMH/Bleomycin Hydrolase Products

Product Documents for BLMH/Bleomycin Hydrolase Antibody (4A2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BLMH/Bleomycin Hydrolase Antibody (4A2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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