Borrelia ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA1468

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum or Plasma, Cerebrospinal Fluid,Synovial Fluid (100 uL (1:100 for serum ; 1:1 for CSF or 1:80 Synovial Fluid for prediluted))

Sensitivity

2.41 AU/mL
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Product Specifications

Assay Type

solid phase

Kit Type

ELISA Kit (Colorimetric)

Description

Borrelia IgM ELISA Kit is a solid phase enzyme immunoassay for the quantitative measurement of IgM autoantibodies against Borrelia.

Precision

Intra-Assay Precision (Precision within an assay) CV% 2.55%

Inter-Assay Precision (Precision between assays) CV% 6.55%

Recovery for Borrelia ELISA Kit (Colorimetric)

Recovery

92% to 107%

Kit Contents for Borrelia ELISA Kit (Colorimetric)

  • 1) r.t.u. = ready to use
  • 2) The antibody concentration for each Standard (A-E) is mentioned in enclosed Quality control certificate AU/mL (Artificial units/mL).
  • Anti-human IgM antibodies labeled with horseradish peroxidase (Px-conjugate) r.t.u.
  • Chromogenic substrate (TMB substrate), r.t.u.
  • Dilution buffer anti-Borrelia rec., r.t.u.
  • ELISA break-away strips coated with specific recombinant antigens
  • Standard A = negative control serum, r.t.u.1), 2)
  • Standard B, r.t.u.
  • Standard C, r.t.u.
  • Standard D = calibrator, r.t.u.
  • Standard E = positive control serum, r.t.u.
  • Stop solution, r.t.u.
  • Wash buffer concentrate, 10x concentrated.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Borrelia

Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.

Additional Borrelia Products

Product Documents for Borrelia ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Borrelia ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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