Bovine Osteopontin (OPN) Protein

Name: Bovine Osteopontin (OPN) Protein
Brand: R&D Systems
SKU: 109-OP
Price: 349 USD
Availability: InStock

R&D Systems | Catalog # 109-OP

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Citations (4)

Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems Bovine Milk-derived Bovine Osteopontin (OPN) Protein (109-OP)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

Bovine Milk

Applications

Bioactivity

View all Osteopontin/OPN Proteins and Enzymes »

Product Specifications

Source

Bovine milk-derived Osteopontin/OPN protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED₅₀ for this effect is < 1.00 µg/mL.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 109-OP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 109-OP/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Osteopontin/OPN

Osteopontin (OPN, also known as early T lymphocyte activation protein 1, bone sialoprotein 1, and secreted phosphoprotein 1), is a secreted, highly acidic,
calcium-binding, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels, and various tumor tissues. Many cell types can express OPN in response to activation by cytokines, growth factors, or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, tumor metastasis, and cerebral ischemia (1, 2). Bovine OPN cDNA encodes a 278 amino acid (aa) residue precursor with a 16 aa signal peptide that is cleaved to yield a 262 aa mature protein with an integrin binding sequence (RGD), a thrombin cleavage site and N- and O-glycosylation sites. Human, mouse, rat, porcine, and bovine OPN share approximately 40%-80% aa sequence identity. OPN has been shown to bind to different cell types through RGD-mediated interaction with Integrins alpha _v beta ₁, alpha _v beta ₃, alpha _v beta ₅, and non-RGD-mediated interaction with CD44 and Integrins alpha ₈ beta ₁or alpha ₉ beta ₁. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3, and MMP-7. The functions of OPN in a variety of cell types are modified as a result of proteolytic cleavage (3, 4).