Bradykinin RB1/BDKRB1 Antibody - BSA Free

Novus Biologicals | Catalog # NLS3580

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bradykinin RB1/BDKRB1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 18 amino acid peptide from C-terminus of human Bradykinin RB1/BDKRB1.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Bovine (89%), Equine (89%), Porcine (83%).

Localization

Cell membrane

Specificity

Human BDKRB1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Bradykinin RB1/BDKRB1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody - BSA Free [NLS3580]

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody - BSA Free [NLS3580]

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS3580] - Human Vascular Smooth Muscle: Formalin-Fixed, paraffin-Embedded (FFPE)
Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody - BSA Free [NLS3580]

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody - BSA Free [NLS3580]

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS3580] - Analysis of anti-BDKRB1 antibody with nasal mucosa at 24 ug/ml.
Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody - BSA Free [NLS3580]

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody - BSA Free [NLS3580]

Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS3580] - Analysis of anti-BDKRB1 antibody with human placenta.

Applications for Bradykinin RB1/BDKRB1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 - 15 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Bradykinin RB1/BDKRB1

Bradykinin Receptor B1 (BKB1) has been implicated in inflammation and nociception. BKB1 receptor is rapidly induced under many inflammatory conditions such as colitis and hyperalgesia, allergic rhinitis, and inflammation. In vitro studies have implicated lipopolysaccharide, interleukins, and interferon as effectors in the rapid induction of BKB1 receptor expression. BKB1 receptor-deficient mice are healthy, fertile, and normotensive but show blunted response to lipopolysaccharide-induced hypotension, reduced migration into inflamed tissues by polymorphonuclear leukocytes, and reduced activity-dependent facilitation of a spinal nociceptive reflex. Bradykinin B1 receptor is generally only minimally expressed under normal physiological conditions, but the expression is induced rapidly under pathologic conditions such as ischemia, atheromatous disease, or exposure to inflammatory cytokines. Expression has been reported in brain, colon, kidney, lung, nasal mucosa, pancreas, prostate, skin, spinal cord, and vessel. ESTs have been isolated from normal human connective tissue libraries and in human kidney and skin cancer libraries.

Long Name

Bradykinin Receptor B1

Alternate Names

B1BKR, BDKRB1, BK-1 Receptor, BKB1R, BKR1, BRADYB1, BradykininRB1

Entrez Gene IDs

623 (Human)

Gene Symbol

BDKRB1

UniProt

P46663 (Human)

Additional Bradykinin RB1/BDKRB1 Products

Product Documents for Bradykinin RB1/BDKRB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bradykinin RB1/BDKRB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for Bradykinin RB1/BDKRB1 Antibody - BSA Free (NLS3580):

Immunohistochemistry Protocol for BDKRB1 Antibody (NLS3580):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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