Human Brain Whole Tissue Lysate (Adult Whole Normal)

A: Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

A: You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

A: We recommend loading 10-20ug of lysate per well for Western blot.

A: Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

A: Please see this link to all of our normal human brain tissue lysates.

A: Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

A: You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

A: We recommend loading 10-20ug of lysate per well for Western blot.

A: Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

A: Please see this link to all of our normal human brain tissue lysates.

A: Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

A: You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

A: We recommend loading 10-20ug of lysate per well for Western blot.

A: Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

A: Please see this link to all of our normal human brain tissue lysates.

A: Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

A: You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

A: We recommend loading 10-20ug of lysate per well for Western blot.

A: Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

A: Please see this link to all of our normal human brain tissue lysates.

A: Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

A: You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

A: We recommend loading 10-20ug of lysate per well for Western blot.

A: Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

A: Please see this link to all of our normal human brain tissue lysates.