BRCA2 Antibody (BRCA2/2158)

Novus Biologicals | Catalog # NBP3-23660

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BRCA2/2158
View all BRCA2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 200-500) of human BRCA2 protein (exact sequence is proprietary)

Localization

Nucleus. Cytoplasm.

Marker

Breast Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for BRCA2 Antibody (BRCA2/2158)

BRCA2 Antibody (BRCA2/2158) Immunohistochemistry-Paraffin: BRCA2 Antibody (BRCA2/2158) [NBP3-23660] -

Immunohistochemistry-Paraffin: BRCA2 Antibody (BRCA2/2158) [NBP3-23660] -

Immunohistochemistry-Paraffin: BRCA2 Antibody (BRCA2/2158) [NBP3-23660] - Formalin-fixed, paraffin-embedded human salivary gland stained with BRCA2 Antibody (BRCA2/2158). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for BRCA2 Antibody (BRCA2/2158)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: BRCA2

BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.

Long Name

Breast Cancer 2

Alternate Names

BRCA1/BRCA2-containing complex, subunit 2, BRCC2, breast and ovarian cancer susceptibility gene, early onset, breast cancer 2 tumor suppressor, breast cancer 2, early onset, breast cancer susceptibility protein BRCA2, breast cancer type 2 susceptibility protein, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1Fanconi anemia, complementation group D1, Fanconi anemia group D1 protein, GLM3, PNCA2

Gene Symbol

BRCA2

Additional BRCA2 Products

Product Documents for BRCA2 Antibody (BRCA2/2158)

Certificate of Analysis

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Product Specific Notices for BRCA2 Antibody (BRCA2/2158)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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