Brk Antibody (2H12B8)

Name: Brk Antibody (2H12B8)
Brand: Novus Biologicals
SKU: NB110-60537
Price: 439 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NB110-60537

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 2H12B8

View all Brk Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Brk expressed in E. Coli.

Specificity

PTK6 (2H12B8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Brk Antibody (2H12B8) (NB110-60537) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Brk Antibody (2H12B8)

Western Blot: Brk Antibody (2H12B8)BSA Free [NB110-60537]

Western Blot: Brk Antibody (2H12B8) [NB110-60537] - Analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.

Western Blot: Mouse Monoclonal Brk Antibody (2H12B8) [NB110-60537]

Western Blot: Mouse Monoclonal Brk Antibody (2H12B8) [NB110-60537] - Whole cell lysates from MDA-MB-231 cells that were exposed to 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. Brk Antibody (NB110-60537) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Applications for Brk Antibody (2H12B8)

Application

Recommended Usage

ELISA

1:10000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NB110-60537 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brk

PTK6 (protein tyrosine kinase 6, BRK or FLJ42088),with 451-amino acid protein (about 51kDa), encods a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphory-lation. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. And Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes, but not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Over expression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.

Long Name

Breast Tumor Kinase

Alternate Names

PTK6, Sik, Tksk

Entrez Gene IDs

5753 (Human)

Gene Symbol

PTK6

Additional Brk Products

Product Documents for Brk Antibody (2H12B8)

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Download SDS

Product Specific Notices for Brk Antibody (2H12B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Intracellular Kinases

Customer Reviews for Brk Antibody (2H12B8) (1)

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1 Customer Rating

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Brk Antibody (2H12B8) - BSA Free

Name: Yongkang Yang

Application: Western Blot

Sample Tested: human MDAMB-231 whole cell lysate

Species: Human

Verified Customer | Posted 11/27/2024

Western Blot: whole cell lysates from MDA-MB-231 cells that were exposed to 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. Brk Antibody (NB110-60537) was used for primary antibody: 1:1000, 4℃, overnight.

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