Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

Novus Biologicals | Catalog # NBP2-44055

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 29G6.E8

Format

BSA Free
View all Bromodeoxyuridine/BrdU Primary Antibodies »

Product Specifications

Immunogen

Bromodeoxyuridine/Bromodeoxyuridine/BrdU antibody (29G6.E8) was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.

Specificity

This antibody reacts strongly with Bromodeoxyuridine/BrdU. Cross-reactivity is observed with CldU and IdU.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

This antibody Monoclonal Antibody was purified from ascites fluid by Protein A chromatography

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

Western Blot: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Western Blot: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Western Blot: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 Ug per lane.
Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing hindbrain. Fixation: 4% PFA. Antigen retrieval: not required. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.
Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required.
Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4C. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.

Applications for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:10000

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:500-1:3000

Immunohistochemistry

1:100-1:500

Western Blot

1:2000-1:5000
Application Notes
This product has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Citations for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

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Protocols

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FAQs for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

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  • Q: I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5

    A:

    We recommend this product: NB500-235

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