Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Janelia Fluor 646

Antibody Source

Polyclonal Sheep
View all BromoTag Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to the second bromodomain of Brd4 with a L387A mutation (see Bond, et al PMID 34652918).

Clonality

Polyclonal

Host

Sheep

Applications for BromoTag Antibody [Janelia Fluor® 646]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: BromoTag

BromoTag is a novel TAG degradation platform designed to degrade intracellular proteins of interest. The BromoTag system requires the target protein to be expressed as a fusion protein with a TAG protein, called a degron tag. The BromoTag consists of a bromo and extracellular terminal (BET) bromodomain Brd4 variant L387A as the degron tag. The tag mediates formation of a tertiary complex between the von Hippel Lindau (VHL) E3 ligase and the fusion protein. The fusion protein becomes ubiquitinated by the E3 ligase, leading to proteasomal degradation of the target protein and degrader BromoTag recycling. An advantage of this system is that the degron tag removes the requirement for proteolysis targeting chimeras (PROTACs) or binding ligands for the protein of interest to recruit the E3 ubiquitin ligase. Degradation by BromoTag is an alternative strategy compared to conventional genetic knockout or knockdown. Additionally, the dosage can be modified to control the level of protein degradation and knockdown. The Bromotag system is also reversible, where in cell culture conditions the degrader can be washed out from the media.

BromoTag(R) is a registered trademark of the University of Dundee and is used under license.

References

Bond AG, Craigon C, Chan KH, et al. Development of BromoTag: A "Bump-and-Hole"-PROTAC System to Induce Potent, Rapid, and Selective Degradation of Tagged Target Proteins. J Med Chem. 2021;64(20):15477-15502. https://doi.org/10.1021/acs.jmedchem.1c01532

Alternate Names

Brd4 bromodomain L387A variant, Brd4BD2L387A, BromoTag degron

Additional BromoTag Products

Product Documents for BromoTag Antibody [Janelia Fluor® 646]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BromoTag Antibody [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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