BTN3A1 Antibody (CTX-2026) - Humanized - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP3-28506

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # CTX-2026 expressed in CHO

Format

Low Endotoxin, Azide and BSA Free
View all CD277/BTN3A1 Primary Antibodies »

Product Specifications

Immunogen

BTN3A1

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Patent #: US20210309746

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Scientific Data Images

BTN2A1 Antibody (CTX-2026) - Humanized Flow Cytometry: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] -

Flow Cytometry: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] -

Flow Cytometry: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] - Human BTN3A1 HEK293 cells were stained with Anti-BTN3A1 Reference Antibody (CTX-2026) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.8254 nM.
BTN2A1 Antibody (CTX-2026) - Humanized ELISA: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] -

ELISA: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] -

ELISA: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] - Immobilized human PVRIG His at 2 ug/mL can bind Anti-BTN3A1 Reference Antibody (CTX-2026), EC50=0.002394 ug/mL.
BTN2A1 Antibody (CTX-2026) - Humanized Surface Plasmon Resonance: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] -

Surface Plasmon Resonance: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] -

Surface Plasmon Resonance: BTN3A1 Antibody (CTX-2026) - Humanized [NBP3-28506] - Anti-BTN3A1 Reference Antibody（CTX-2026）captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human BTN3A1, His Tag with an affinity constant of 9.89E-11M as determined in a SPR assay.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD277/BTN3A1

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]

Alternate Names

BT3.1, BTF5, BTN3A1, CD277

Gene Symbol

BTN3A1

UniProt

Q13410 (Human)

Additional CD277/BTN3A1 Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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