Bub1 Antibody (1A11C7) - BSA Free

Novus Biologicals | Catalog # NBP2-61719

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A11C7

Format

BSA Free
View all Bub1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Bub1 (AA: 1-130) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bub1 Antibody (1A11C7) - BSA Free

Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)
Flow Cytometry: Bub1 Antibody (1A11C7) - BSA Free [NBP2-61719]

Flow Cytometry: Bub1 Antibody (1A11C7) - BSA Free [NBP2-61719]

Flow Cytometry: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).
Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Bub1 Antibody (1A11C7) - BSA Free [NBP2-61719]

ELISA: Bub1 Antibody (1A11C7) - BSA Free [NBP2-61719]

ELISA: Bub1 Antibody (1A11C7) [NBP2-61719] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Bub1 Antibody (1A11C7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bub1

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.;

Long Name

BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase

Gene Symbol

BUB1

Additional Bub1 Products

Product Documents for Bub1 Antibody (1A11C7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bub1 Antibody (1A11C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Bub1 Antibody (1A11C7) - BSA Free

There are currently no reviews for this product. Be the first to review Bub1 Antibody (1A11C7) - BSA Free and earn rewards!

Have you used Bub1 Antibody (1A11C7) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies