Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2H6

Format

BSA Free
View all Bub3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human BUB3 (1-328aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Bub3 Antibody (2H6) - BSA Free

Western Blot: Bub3 Antibody (2H6)BSA Free [NBP2-42680]

Western Blot: Bub3 Antibody (2H6)BSA Free [NBP2-42680]

Western Blot: Bub3 Antibody (2H6) [NBP2-42680] - The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for Bub3 Antibody (2H6) - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bub3

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Long Name

BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog (yeast), hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3

Gene Symbol

BUB3

UniProt

NP_004716 (Human)

Additional Bub3 Products

Product Documents for Bub3 Antibody (2H6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Bub3 Antibody (2H6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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