Home / BoNT-B Light Chain / C. botulinum BoNT-B Light Chain Antibody

C. botulinum BoNT-B Light Chain Antibody

Catalog #: AF5420
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5420
AF5420-SP

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Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-B Light Chain Antibody Summary

Species Reactivity
C. botulinum
Specificity
Detects C. botulinum BoNT-B Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant Light Chains of BoNT-A, -E, or recombinant Heavy Chains of BoNT-C, or -D is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant Clostridium BoNT-B Light Chain
Pro2-His428
Accession # P10844
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Botulinum Neurotoxin Type B Light Chain (Catalog # 5420-ZN)
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant Botulinum Neurotoxin Type B Light Chain (Catalog # 5420-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-B Light Chain

Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.

References
  1. Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
  2. Montecucco, C. and Giampietro, S. (1993) Trends Biochem. Sci. 18:324.
  3. Turton, K. et al. (2002) Trends Biochem. Sci. 27:552.
  4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.
Long Name
Botulinum Neurotoxin Type B Light Chain
Entrez Gene IDs
6030102 (C. botulinum)
Alternate Names
BoNTB Light Chain; BoNT-B Light Chain; DcD

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Citations for C. botulinum BoNT-B Light Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction
    Authors: M Golan, J Boulanger-, A Pinot, P Fontanaud, A Faucherre, DS Gajbhiye, L Hollander-, T Fiordelisi, AO Martin, P Mollard
    Science Advances, 2021-02-19;7(8):.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC
  2. SERS detection of Clostridium botulinum neurotoxin serotypes A and B in buffer and serum: Towards the development of a biodefense test platform
    Authors: China Y. Lim, Jennifer H. Granger, Marc D. Porter
    Analytica Chimica Acta: X
  3. Oligodendrocyte-lineage cell exocytosis and L-type prostaglandin D synthase promote oligodendrocyte development and myelination
    Authors: L Pan, A Trimarco, AJ Zhang, K Fujimori, Y Urade, LO Sun, C Taveggia, Y Zhang
    Elife, 2023-02-13;12(0):.

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