C. botulinum BoNT-B Light Chain Antibody
C. botulinum BoNT-B Light Chain Antibody Summary
Pro2-His428
Accession # P10844
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BoNT-B Light Chain
Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.
- Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
- Montecucco, C. and Giampietro, S. (1993) Trends Biochem. Sci. 18:324.
- Turton, K. et al. (2002) Trends Biochem. Sci. 27:552.
- Schiavo, G. et al. (2000) Physiol. Rev. 80:717.
Product Datasheets
Citation for C. botulinum BoNT-B Light Chain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction
Authors: M Golan, J Boulanger-, A Pinot, P Fontanaud, A Faucherre, DS Gajbhiye, L Hollander-, T Fiordelisi, AO Martin, P Mollard
Science Advances, 2021;7(8):.
Species: Zebrafish
Sample Types: Whole Tissue
Applications: IHC
FAQs
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