Key Product Details

Validated by

Biological Validation

Species Reactivity

Sheep

Applications

Control

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Normal Sheep IgG Control

Detection of Normal Sheep IgG Control by Control

Detection of Normal Sheep IgG Control by Control

Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Normal Sheep IgG Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 7 reviews rated 4.7 using 5-001-A in the following applications:

Formulation, Preparation, and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Normal Sheep IgG Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Normal Sheep IgG Control

For research use only

Citations for Normal Sheep IgG Control

Customer Reviews for Normal Sheep IgG Control (7)

4.7 out of 5
7 Customer Ratings
5 Stars
71%
4 Stars
29%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • Normal Sheep IgG Control
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/22/2019
    Normal Sheep IgG Control 5-001-A
  • Normal Sheep IgG Control
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/04/2019
  • Normal Sheep IgG Control
    Name: avinash waghray
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 03/11/2019
    no background - with and without retrieval
    Normal Sheep IgG Control 5-001-A
  • Normal Sheep IgG Control
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Human recombinant test antibody and Human recombinant antibody
    Species: Mouse and Human
    Verified Customer | Posted 05/04/2018
  • Normal Sheep IgG Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Cancer Cells
    Species: Human
    Verified Customer | Posted 02/20/2018
    Normal Sheep IgG Control 5-001-A
  • Normal Sheep IgG Control
    Name: Anonymous
    Verified Customer | Posted 02/14/2018
    For surface staining of PANC1 cells as Isotype control along with Anti sheep Alexa 647 secondary Antibody
    Normal Sheep IgG Control 5-001-A
  • Normal Sheep IgG Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 09/27/2017
    Great antibody control for ICC
    Normal Sheep IgG Control 5-001-A

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FAQs

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