Sheep IgG NorthernLights™ NL557-conjugated Antibody

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL010

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Detection of Sheep IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (4)

Sheep IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity
Sheep
Specificity
Sheep IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Sheep IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Use at a 1:200 dilution
 
Immunohistochemistry
Use at a 1:200 dilution
 
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Sheep IgG Primary Antibody by Immunocytochemistry. Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in KG-1 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Sheep IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Systematic single-cell pathway analysis to characterize early T�cell activation
    Authors: JA Bibby, D Agarwal, T Freiwald, N Kunz, NS Merle, EE West, P Singh, A Larochelle, F Chinian, S Mukherjee, B Afzali, C Kemper, NR Zhang
    Cell Reports, 2022-11-22;41(8):111697.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
    Authors: W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun
    Nature Immunology, 2022-07-06;23(7):1021-1030.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days
    Authors: N Won, J Castillo-P, X Tan, J Ford, D Heath, LI Mazilescu, M Selzner, IM Rogers
    Cells, 2022-06-02;11(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration
    Authors: P Flora, MY Li, PM Galbo, M Astorkia, D Zheng, E Ezhkova
    PloS Genetics, 2021-12-14;17(12):e1009948.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
    Authors: K Karnas, T Str?czek, C Kapusta, M Lekka, J Duli?ska-L, A Karewicz
    International Journal of Nanomedicine, 2021-09-24;16(0):6537-6552.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
    Authors: EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
    Cell Stem Cell, 2020-09-17;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Pathogenesis of hypertension in a mouse model for human CLCN2 related hyperaldosteronism
    Authors: C Göppner, IJ Orozco, MB Hoegg-Beil, AH Soria, CA Hübner, FL Fernandes-, S Boulkroun, MC Zennaro, TJ Jentsch
    Nat Commun, 2019-10-15;10(1):4678.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. IFN-I and IL-22 mediate protective effects of intestinal viral infection
    Authors: JA Neil, Y Matsuzawa-, E Kernbauer-, SL Schuster, S Sota, M Venzon, S Dallari, A Galvao Net, A Hine, D Hudesman, P Loke, TJ Nice, K Cadwell
    Nat Microbiol, 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: IHC
  9. Role of Nesfatin-1 in the Reproductive Axis of Male Rat
    Sci Rep, 2016-09-07;6(0):32877.
    Applications: IHC
  10. Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
    Authors: Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M
    Stem Cells Int, 2014-09-10;2014(0):468927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  11. Antitumor effects of chimeric receptor engineered human T cells directed to tumor stroma.
    Authors: Kakarla S, Chow K, Mata M, Shaffer D, Song X, Wu M, Liu H, Wang L, Rowley D, Pfizenmaier K, Gottschalk S
    Mol Ther, 2013-06-04;21(8):1611-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: CAR-T (IHC-P)
  12. Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease.
    Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
    PLoS ONE, 2012-06-07;7(6):e38615.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
    Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
    Science, 2010-11-05;330(6005):827-30.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Sheep IgG NorthernLights™ NL557-conjugated Antibody

Average Rating: 4.3 (Based on 4 Reviews)

5 Star
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4 Star
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody
By Danlee Enzler on 05/01/2018
Application: IHC Sample Tested: Liver tissue Species: Donkey

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody
By Anonymous on 09/27/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody
By Anonymous on 12/23/2016

Used with the Human CD109 antibody from R&D Systems.


Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody
By Anonymous on 07/27/2016
Application: Flow Sample Tested: Sheep IgG Species: Sheep