Sheep IgG NorthernLights™ NL637-conjugated Antibody

NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
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Detection of Sheep IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (5)
Supplemental Products
Reviews (1)

Sheep IgG NorthernLights™ NL637-conjugated Antibody Summary

Species Reactivity
Sheep IgG (H+L)
Polyclonal Donkey IgG
Antigen Affinity-purified
Sheep IgG
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
NL637 (Excitation= 637 nm, Emission= 658 nm)


Recommended Concentration
Flow Cytometry
Use at a 1:200 dilution
Use at a 1:200 dilution
Use at a 1:200 dilution

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Sheep IgG Primary Antibody by Immunocytochemistry. Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in KG-1 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Sheep IgG NorthernLights™ NL637-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Plexin B2 and Semaphorin 4C Guide T Cell Recruitment and Function in the Germinal Center
    Authors: H Yan, L Wu, C Shih, S Hou, J Shi, T Mao, W Chen, B Melvin, RJ Rigby, Y Chen, H Jiang, RH Friedel, CG Vinuesa, H Qi
    Cell Rep, 2017-05-02;19(5):995-1007.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Proc Natl Acad Sci USA, 2016-07-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Scavenger receptor-BI is a receptor for lipoprotein(a).
    Authors: Yang X, Amar M, Vaisman B, Bocharov A, Vishnyakova T, Freeman L, Kurlander R, Patterson A, Becker L, Remaley A
    J Lipid Res, 2013-06-29;54(9):2450-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm.
    Authors: Trichas G, Joyce B, Crompton LA, Wilkins V, Clements M, Tada M, Rodriguez TA, Srinivas S
    PLoS Biol., 2011-02-22;9(2):e1001019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Secondary Antibody
  5. c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
    Authors: Szabo R, Rasmussen AL, Moyer AB
    Oncogene, 2011-01-10;30(17):2003-16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Sheep IgG NorthernLights™ NL637-conjugated Antibody

Average Rating: 4 (Based on 1 Review)

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody
By Anonymous on 07/27/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Lung tissue Species: Sheep