Detection of SheepIgG Primary Antibody by Flow Cytometry HeLa human cervical epithelial carcinoma cell line wasstained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody|
(Catalog # AF6185,filled histogram) or isotype control antibody (Catalog # 5-001-A,open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG SecondaryAntibody (Catalog # F0126) at 10 μL/106 cells. To facilitateintracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.