Sheep IgG (H+L) PE-conjugated Antibody

  
  • Species Reactivity
    Sheep
  • Specificity
    Sheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity.
  • Source
    Polyclonal Donkey IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Sheep IgG
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SheepIgG Primary Antibody by Flow Cytometry HeLa human cervical epithelial carcinoma cell line wasstained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF6185,filled histogram) or isotype control antibody (Catalog # 5-001-A,open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG SecondaryAntibody (Catalog # F0126) at 10 μL/106 cells.  To facilitateintracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
View all Antibody FAQs
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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