Sheep IgG (H+L) PE-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SheepIgG Primary Antibody by Flow Cytometry
HeLa human cervical epithelial carcinoma cell line wasstained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF6185,filled histogram) or isotype control antibody (Catalog # 5-001-A,open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG SecondaryAntibody (Catalog # F0126) at 10 μL/106 cells. To facilitateintracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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