Sheep IgG (H+L) PE-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Sheep IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (1)
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Sheep IgG (H+L) PE-conjugated Antibody Summary

Species Reactivity
Sheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity.
Polyclonal Donkey IgG
Antigen Affinity-purified
Sheep IgG
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Sheep IgG Primary Antibody by Flow Cytometry View Larger

Detection of Sheep IgG Primary Antibody by Flow Cytometry HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) at 10 µL/106cells. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citation for Sheep IgG (H+L) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. WAIF1 is a cell-surface CTHRC1 binding protein coupling bone resorption and formation
    Authors: K Matsuoka, Y Kohara, Y Naoe, A Watanabe, M Ito, K Ikeda, S Takeshita
    J. Bone Miner. Res., 2018-05-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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