Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of SheepIgG Primary Antibody by Flow Cytometry
HeLa human cervical epithelial carcinoma cell line wasstained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185,filled histogram) or isotype control antibody (Catalog # 5-001-A,open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG SecondaryAntibody (Catalog # F0126) at 10 μL/106 cells. To facilitateintracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
Have you used Sheep IgG (H+L) PE-conjugated Antibody?
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