C. botulinum BoNT‑D Light Chain Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB6037AFP680

R&D Systems

Key Product Details

Species Reactivity

C. botulinum

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 695004
View all BoNT-D Light Chain Primary Antibodies »

Product Specifications

Specificity

Detects C. botulinum BoNT-D Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant Light Chains of BoNT-A, -B, -C1, -E, -F, -G or recombinant Heavy Chains of BoNT-A, -C1, or -D is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BoNT-D Light Chain

BoNT-D LC (Botulinum neurotoxin serotype D light chain) is a 50-55 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits acetylcholine release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Notably, BoNT-D is not toxic to human cells. BoNT/D precursor is 1276 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that shows a disulfide-linked 442 aa light chain/enzyme N-terminus, and a 834 aa heavy chain/receptor-binding C-terminus. The 100 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. Covalent attachment of cargo molecules to the N-terminus is used to transport molecules into the cytosol. The D light chain shares less that 50% aa identity with the other botulinum serotype light chains.        

Long Name

Botulinum Neurotoxin Type D Light Chain

Alternate Names

BoNTD Light Chain

UniProt

P19321 (C. botulinum)

Additional BoNT-D Light Chain Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Metalloproteases and Regulators

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