C. botulinum BoNT-D Light Chain Antibody

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Product Details
Supplemental Products

C. botulinum BoNT-D Light Chain Antibody Summary

Species Reactivity
C. botulinum
Detects C. botulinum BoNT-D Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant Light Chains of BoNT-A, -B, -C1, -E, -F, -G or recombinant Heavy Chains of BoNT-A, -C1, or -D is observed.
Monoclonal Mouse IgG1 Clone # 695004
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant C. botulinum BoNT-D Light Chain
Accession # P19321
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant C. botulinum BoNT-D Light Chain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-D Light Chain

BoNT-D LC (Botulinum neurotoxin serotype D light chain) is a 50-55 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits acetylcholine release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Notably, BoNT-D is not toxic to human cells. BoNT/D precursor is 1276 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that shows a disulfide-linked 442 aa light chain/enzyme N-terminus, and a 834 aa heavy chain/receptor-binding C-terminus. The 100 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. Covalent attachment of cargo molecules to the N-terminus is used to transport molecules into the cytosol. The D light chain shares less that 50% aa identity with the other botulinum serotype light chains.        

Long Name
Botulinum Neurotoxin Type D Light Chain
Alternate Names
BoNTD Light Chain; BoNT-D Light Chain

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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