Key Product Details

Species Reactivity

C. botulinum

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 695004
View all BoNT-D Light Chain Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant C. botulinum BoNT-D Light Chain
Thr2-Ser428
Accession # P19321

Specificity

Detects C. botulinum BoNT-D Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant Light Chains of BoNT-A, -B, -C1, -E, -F, -G or recombinant Heavy Chains of BoNT-A, -C1, or -D is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for C. botulinum BoNT‑D Light Chain Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant C. botulinum BoNT-D Light Chain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BoNT-D Light Chain

BoNT-D LC (Botulinum neurotoxin serotype D light chain) is a 50-55 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits acetylcholine release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Notably, BoNT-D is not toxic to human cells. BoNT/D precursor is 1276 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that shows a disulfide-linked 442 aa light chain/enzyme N-terminus, and a 834 aa heavy chain/receptor-binding C-terminus. The 100 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. Covalent attachment of cargo molecules to the N-terminus is used to transport molecules into the cytosol. The D light chain shares less that 50% aa identity with the other botulinum serotype light chains.        

Long Name

Botulinum Neurotoxin Type D Light Chain

Alternate Names

BoNTD Light Chain

UniProt

P19321 (C. botulinum)

Additional BoNT-D Light Chain Products

Product Documents for C. botulinum BoNT‑D Light Chain Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for C. botulinum BoNT‑D Light Chain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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