Q: I would like to use ELISA kit NBP2-66708 to find the C5b-9 concentration in human plasma. How do you make the C5b-9 reference standard?

A: We have tested this kit with human plasma samples and the reference value is about 100~500ng/mL. The reference standard applied for this item is purified natural human SC5b9. Unfortunately we cannot provide its commercial resource because of proprietary reasons.

Human C5b-9 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-66708

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Supernatant, Tissue Homogenate (100 uL)

Sensitivity

18.75 ng/mL

Assay Range

31.25 - 2000 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human sC5b9 (soluble terminal complement complex C5b-9) in samples. No significant cross-reactivity or interference between Human sC5b9 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 4.96

Inter-Assay Precision (Precision between assays) %CV 5.07

Recovery for Human C5b-9 ELISA Kit (Colorimetric)

Recovery

87 - 101%

Linearity

Scientific Data Images for Human C5b-9 ELISA Kit (Colorimetric)

ELISA: Human C5b-9 ELISA Kit (Colorimetric) [NBP2-66708] -

ELISA: Human C5b-9 ELISA Kit (Colorimetric) [NBP2-66708] - Standard Curve Reference

Kit Contents for Human C5b-9 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: C5b-9

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Alternate Names

MAC, Membrane attack complex, sC5-b9, sC5b-9, TCC, Terminal complement complex

Additional C5b-9 Products

Product Documents for Human C5b-9 ELISA Kit (Colorimetric)

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Download Human C5b-9 ELISA Kit (Colorimetric) Manual

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human C5b-9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human C5b-9 ELISA Kit (Colorimetric)

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