CA125/MUC16 Antibody (X306cc) - BSA Free
Novus Biologicals | Catalog # NB120-10032
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Format
Product Specifications
Immunogen
Epitope
Localization
Specificity
Clonality
Host
Isotype
Applications for CA125/MUC16 Antibody (X306cc) - BSA Free
ELISA
Sandwich ELISA
Simple Western
Western Blot
See Simple Western Antibody Database for Simple Western validation: separated by Size, antibody dilution of 1:50, apparent MW was 66-440 kDa
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: CA125/MUC16
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional CA125/MUC16 Products
Product Documents for CA125/MUC16 Antibody (X306cc) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CA125/MUC16 Antibody (X306cc) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CA125/MUC16 Antibody (X306cc) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CA125/MUC16 Antibody (X306cc) - BSA Free
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Q: I need to order 3 batches of this antibody with a concentration near to 9 mg/mL, and I would like to know if it is possible to receive the product with this concentration?
A: We unfortunately do not have any lots ready that are available at 9.0mg/ml. This is what we have currently: Lot: 12/07-C29-X306, conc. 4.1 mg/ml, Lot: 12/08-C29-X306, conc. 6.4 mg/ml, Lot: 13/01-C29-X306, conc. 3.1 mg/ml. We have our productions purified off site and receive the units in at their given concentrations. Unless you are interested in purchasing a bulk amount, we will only be able offer you the product we have currently available.
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Q: We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?
A: These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.
-
Q: I need to order 3 batches of this antibody with a concentration near to 9 mg/mL, and I would like to know if it is possible to receive the product with this concentration?
A: We unfortunately do not have any lots ready that are available at 9.0mg/ml. This is what we have currently: Lot: 12/07-C29-X306, conc. 4.1 mg/ml, Lot: 12/08-C29-X306, conc. 6.4 mg/ml, Lot: 13/01-C29-X306, conc. 3.1 mg/ml. We have our productions purified off site and receive the units in at their given concentrations. Unless you are interested in purchasing a bulk amount, we will only be able offer you the product we have currently available.
-
Q: We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?
A: These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.