![Immunohistochemistry-Paraffin: CA15-3 Antibody (DBMM1) [NBP2-26672]](https://resources.rndsystems.com/images/products/CA15-3-Antibody-DBMM1-Immunohistochemistry-Paraffin-NBP2-26672-img0001.jpg "Immunohistochemistry-Paraffin: CA15-3 Antibody (DBMM1) [NBP2-26672]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DBMM1
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Product Specifications
Immunogen
Human breast cancer cell line ZR-75-1
Reactivity Notes
Human
Localization
Cytoplasmic, cell membrane
Specificity
This antibody is specific to epithelial membrane antigen (EMA), CA15-3, or polymorphic epithelial mucin. This antibody stains an underglycosylated MUC1 often present on carcinoma cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CA15-3 Antibody (DBMM1)
Immunohistochemistry-Paraffin: CA15-3 Antibody (DBMM1) [NBP2-26672]
Immunohistochemistry-Paraffin: CA15-3 Antibody (DBMM1) [NBP2-26672] - Formalin fixed paraffin embedded, stained with CA15-3 Antibody (NBP2-26672).Applications for CA15-3 Antibody (DBMM1)
Application
Recommended Usage
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Application Notes
IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CA15-3/MUC-1
Long Name
Cancer Antigen 15-3
Alternate Names
CA153, MUC1
Additional CA15-3/MUC-1 Products
Product Documents for CA15-3 Antibody (DBMM1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CA15-3 Antibody (DBMM1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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