Cadherin-16 Antibody (CDH16/7027R)

Novus Biologicals | Catalog # NBP3-13828

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # CDH16/7027R
View all Cadherin-16 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-length Cadherin-16 protein (Uniprot: O75309 )

Localization

Cell surface, Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14213)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cadherin-16 Antibody (CDH16/7027R)

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828] - IHC analysis of formalin-fixed, paraffin-embedded human kidney. Staining using Cadherin-16 antibody (CDH16/7027R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Staining using Cadherin-16 antibody (CDH16/7027R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828] - Formalin-fixed, paraffin-embedded human kidney stained with Cadherin-16 antibody (CDH16/7027R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Cadherin-16 Antibody (CDH16/7027R) Western Blot: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828] -

Western Blot: Cadherin-16 Antibody (CDH16/7027R) [NBP3-13828] -

Western Blot Analysis of human kidney tissue lysate using Cadherin-16 Antibody (CDH16/7027R).

Applications for Cadherin-16 Antibody (CDH16/7027R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cadherin-16

Cadherin 16 is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development.

Alternate Names

cadherin 16, KSP-cadherin, cadherin-16, Kidney-specific cadherin, KSP-cadherin

Gene Symbol

CDH16

UniProt

O75309 (Human)

Additional Cadherin-16 Products

Product Documents for Cadherin-16 Antibody (CDH16/7027R)

Certificate of Analysis

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Product Specific Notices for Cadherin-16 Antibody (CDH16/7027R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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