Cadherin-17 Antibody (MSVA-517M)

Novus Biologicals | Catalog # NBP3-57249

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MSVA-517M
View all Cadherin-17 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary) Swissprot Q12864

Localization

Cell Surface and Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A strong CDH17 staining should be seen in all epithelial cells..

Negative Control
Negitve control:Colon: Non-epithelial cells should not show any CDH17 immunostaining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cadherin-17 Antibody (MSVA-517M)

Cadherin-17 Antibody (MSVA-517M)

Immunohistochemistry-Paraffin:Cadherin-17 Antibody (MSVA-517M)NBP3-57249

Colon Colorectal adenocarcinoma with strong CDH17 immunostaining of all tumor cells CDH17 immunohistochemistry.
Cadherin-17 Antibody (MSVA-517M)

Immunohistochemistry-Paraffin:Cadherin-17 Antibody (MSVA-517M)NBP3-57249

Ileum Neuroendocrine tumor with strong CDH17 immunostaining of all tumor cells CDH17 immunohistochemistry.
Cadherin-17 Antibody (MSVA-517M)

Immunohistochemistry-Paraffin:Cadherin-17 Antibody (MSVA-517M)NBP3-57249

Rectum mucosa A strong membranous CDH17 staining occurs in all epithelial cells of the rectum mucosa CDH17 immunohistochemistry.

Applications for Cadherin-17 Antibody (MSVA-517M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cadherin-17

LI Cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Alternate Names

Cadherin17, CDH17

Gene Symbol

CDH17

Additional Cadherin-17 Products

Product Documents for Cadherin-17 Antibody (MSVA-517M)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cadherin-17 Antibody (MSVA-517M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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Protocols

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