Cadherin-4/R-Cadherin Antibody

Novus Biologicals | Catalog # NBP2-27372

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Mammal

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Cadherin-4/R-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Amino acids DYDYLNDWGPRFkkLADMYGGGDD of human Cadherin was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is conserved in most mammals.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cadherin-4/R-Cadherin Antibody

Western Blot: Cadherin-4/R-Cadherin Antibody [NBP2-27372]

Western Blot: Cadherin-4/R-Cadherin Antibody [NBP2-27372]

Western Blot: Cadherin-4/R-Cadherin Antibody [NBP2-27372] - Analysis of R Cadherin in A) human and B) mouse brain lysate using R Cadherin antibody at 3 ug/ml.

Applications for Cadherin-4/R-Cadherin Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cadherin-4/R-Cadherin

Cadherins are a family of transmembrane adhesion receptors that are involved in the formation of cell to cell junctions in a variety of tissues, which is crucial for the development, maintenance and homeostasis of tissue architecture and function. Cadherin expression is regulated spatially as well as temporally. The cadherin superfamily of proteins contains over 100 members. Classical cadherins (e.g., N-, E-, and C-cadherin) consist of five CADs, a single transmembrane helix, and a highly conserved cytosolic domain (koch AW et al., 2004). These cadherins feature a 24-amino acid sequence in the carboxy terminal which is conserved among cadherin family proteins and across animal species; antibody generated against this sequence are termed pan-cadherin antibody. Cadherins depend on calcium for their function: removal of calcium abolishes adhesive activity and renders cadherins vunerable to proteases. The cytoplasmic domain of classical cadherins interacts with beta-catenin, gamma-catenin (also called plakoglobin), and p120 catenin. Ctnnb and Ctnng associate with Ctnna, which links the cadherin-catenin complex to the actin cytoskeleton. Deletion of the cytoplasmic domain destroys these interactions and also eliminates cadherin function. Recently Src, Yes, and Lyn gene products of the Src proto-oncogene family have been found to be responsible for Cadherin phosphorylation (Shapiro L et al., 2009). Cadherins are differentially expressed during development and in adult organs. Since many cell types express multiple Cadherin subclasses simultaneously, it can be inferred that the adhesion properties of individual cells are thus governed by varying the combinations of cadherins. There is also recent evidence to suggest that altered expression of cadherins may be involved in invasion and metastasis of tumor cells (Maret D et al., 2010).

Alternate Names

CAD4, Cadherin4, CDH4, R-Cadherin

Gene Symbol

CDH4

UniProt

P55283

Additional Cadherin-4/R-Cadherin Products

Product Documents for Cadherin-4/R-Cadherin Antibody

Certificate of Analysis

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Product Specific Notices for Cadherin-4/R-Cadherin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Cadherin-4/R-Cadherin Antibody

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