Calcein AM

R&D Systems | Catalog # 4892-010-01

Cell permeable dye used to evaluate cell viability
R&D Systems
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Key Product Details

Features

Calcein AM is a cell-permeant dye that can be used to determine cell viability and/or cytotoxicity in most eukaryotic cells.

Product Summary for Calcein AM

What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for Calcein AM

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Related Research Areas

Cell Cycle

Customer Reviews for Calcein AM (3)

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  • Calcein AM
    Name: Anonymous
    Verified Customer | Posted 02/17/2021
    Robust signal. Cells were treated with drug A for 48 hrs. The fluorescence of Calcein was measured using a plate reader.
    Calcein AM 4892-010-01
  • Calcein AM
    Name: Anonymous
    Verified Customer | Posted 09/17/2018
    Samples were drugged and measured on plate reader after 5 days. The count of florescent cells was plotted against concentration to measure effect
    Calcein AM 4892-010-01
  • Calcein AM
    Name: Anonymous
    Verified Customer | Posted 04/25/2018
    Cell staining with Calcein AM, healthy cells bright under microscope
    Calcein AM 4892-010-01

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