Calcein AM

Cell permeable dye used to evaluate cell viability
Catalog # Availability Size / Price Qty
4892-010-01

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Product Details
Citations (3)
FAQs
Reviews (3)

Calcein AM Summary

Calcein AM is a cell-permeant dye that can be used to determine cell viability and/or cytotoxicity in most eukaryotic cells.

What is Calcein AM?

Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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Citations for Calcein AM

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cytotoxicity of 2D engineered nanomaterials in pulmonary and corneal epithelium
    Authors: M Domanico, A Fukuto, LM Tran, JM Bustamante, PC Edwards, KE Pinkerton, SM Thomasy, LS Van Winkle
    NanoImpact, 2022-05-02;26(0):100404.  2022-05-02
  2. Tubuloids derived from human adult kidney and urine for personalized disease modeling
    Authors: F Schutgens, MB Rookmaaker, T Margaritis, A Rios, C Ammerlaan, J Jansen, L Gijzen, M Vormann, A Vonk, M Viveen, FY Yengej, S Derakhshan, KM de Winter-, B Artegiani, R van Boxtel, E Cuppen, APA Hendrickx, MM van den He, E Heitzer, H Lanz, J Beekman, JL Murk, R Masereeuw, F Holstege, J Drost, MC Verhaar, H Clevers
    Nat. Biotechnol., 2019-03-04;37(3):303-313.  2019-03-04
  3. Vascular-endothelial response to IDH1 mutant fibrosarcoma secretome and metabolite: implications on cancer microenvironment
    Authors: MJ Mao, DE Leonardi
    Am J Cancer Res, 2019-01-01;9(1):122-133.  2019-01-01

FAQs

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Reviews for Calcein AM

Average Rating: 5 (Based on 3 Reviews)

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Calcein AM
By Anonymous on 02/17/2021

Robust signal. Cells were treated with drug A for 48 hrs. The fluorescence of Calcein was measured using a plate reader.


Calcein AM
By Anonymous on 09/17/2018

Samples were drugged and measured on plate reader after 5 days. The count of florescent cells was plotted against concentration to measure effect


Calcein AM
By Anonymous on 04/25/2018

Cell staining with Calcein AM, healthy cells bright under microscope