Calcein AM
Calcein AM Summary
What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for Calcein AM
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Vascular-endothelial response to IDH1 mutant fibrosarcoma secretome and metabolite: implications on cancer microenvironment
Authors: MJ Mao, DE Leonardi
Am J Cancer Res, 2019;9(1):122-133. 2019
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Tubuloids derived from human adult kidney and urine for personalized disease modeling
Authors: F Schutgens, MB Rookmaaker, T Margaritis, A Rios, C Ammerlaan, J Jansen, L Gijzen, M Vormann, A Vonk, M Viveen, FY Yengej, S Derakhshan, KM de Winter-, B Artegiani, R van Boxtel, E Cuppen, APA Hendrickx, MM van den He, E Heitzer, H Lanz, J Beekman, JL Murk, R Masereeuw, F Holstege, J Drost, MC Verhaar, H Clevers
Nat. Biotechnol., 2019;37(3):303-313. 2019
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Robust signal. Cells were treated with drug A for 48 hrs. The fluorescence of Calcein was measured using a plate reader.
Samples were drugged and measured on plate reader after 5 days. The count of florescent cells was plotted against concentration to measure effect
Cell staining with Calcein AM, healthy cells bright under microscope
