Calretinin Antibody

Novus Biologicals | Catalog # NBP2-50029

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
View all Calretinin Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length human Calretinin. [UniProt# P22676]

Specificity

This antibody does not cross-react with the related calcium binding proteins Calbindin D-28K and Parvalbumin.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calretinin Antibody

Western Blot: Calretinin Antibody [NBP2-50029]

Western Blot: Calretinin Antibody [NBP2-50029]

Western Blot: Calretinin Antibody [NBP2-50029] - Western blot analysis of different tissue lysates using chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to calretinin protein. The same blot was simultaneously probed with mouse mAb to alpha-nternexin, dilution 1:10,000 in red that reveals the alpha-internexin protein with apparent molecular weight of 66kDa.
Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029]

Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029]

Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029] - Analysis of rat hippocampus section stained with chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green, and costained with mouse mAb to parvalbumin, 1:1,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The calretinin and parvalbumin antibodies label different classes of interneurons.
Calretinin Antibody

Immunohistochemistry: Calretinin Antibody [NBP2-50029] - 

nbp2-50029_chicken-polyclonal-calretinin-antibody-2552023153950.jpg

Applications for Calretinin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:1000 - 1:5000
Application Notes
In Western blot, this antibody recognizes a clear band at ~29 kDa in rat brain lysates and it reacts only with Calretinin protein, and not other calcium-binding proteins.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Preservative

0.035% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

UniProt

P22676 (Human)

Additional Calretinin Products

Product Documents for Calretinin Antibody

Certificate of Analysis

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Product Specific Notices for Calretinin Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calretinin Antibody

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Protocols

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