Calmodulin kinase II (CaMKII) is an 8 - 12 subunit multimeric calcium/calmodulin dependent Ser/Thr protein kinase. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes (alpha, beta, gamma, delta), each of which contains a catalytic, autoregulatory, and subunit-association domain.
CaM Kinase II Antibody (GT4811) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13508
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (92%), Chicken (98%), Porcine (100%), Rabbit (99%), Xenopus (94%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT4811
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human CaM Kinase II. The exact sequence is proprietary.
Reactivity Notes
Rat (80%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for CaM Kinase II Antibody (GT4811) - Azide and BSA Free
Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508]
Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody [GT4811] (NBP3-13508) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508]
Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody (NBP3-13508) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508]
Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody (NBP3-13508) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508]
Western Blot: CaM Kinase II Antibody (GT4811) [NBP3-13508] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CaM Kinase II antibody (NBP3-13508) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Applications for CaM Kinase II Antibody (GT4811) - Azide and BSA Free
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CaM Kinase II
Long Name
Calcium/Calmodulin-dependent Protein Kinase II
Alternate Names
CAMK2, CAMK2B
Gene Symbol
CAMK2B
Additional CaM Kinase II Products
Product Documents for CaM Kinase II Antibody (GT4811) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CaM Kinase II Antibody (GT4811) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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