Canine CD34 Alexa Fluor® 700-conjugated Antibody

FAB3346N Datasheet / COA / SDS

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FAB3346N-100UG

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Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.

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Canine CD34 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine CD34 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 1H6

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL‑1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3346N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1