Canine CD34 Antibody

Name: Canine CD34 Antibody
Brand: R&D Systems
SKU: MAB3346
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB3346

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Canine

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Functional Assay, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1H6

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Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34

Specificity

Detects canine CD34 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Canine CD34 Antibody

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.

CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Monoclonal Antibody (Catalog # MAB3346, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Canine CD34 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: CLL‑1390 canine leukocytic round cell neoplasia cell line

Western Blot

McSweeney, P.A. et al. (1998) Blood 91(6):1977. This application was not tested by R&D Systems.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.