Capicua Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33420

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Capicua Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PKRKMRRRSSCSSEPNTPKSAKCEGDIFTFDRTGTEAEDVLGELEYDKVPYSSLRRTLDQRRALVMQLFQDHGFFPSAQATAAFQARYADIFPSKVCLQLKIREVRQKIMQA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Capicua Antibody - BSA Free

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues. Corresponding CIC RNA-seq data are presented for the same tissues.
Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CIC antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented
Immunocytochemistry/ Immunofluorescence: Capicua Antibody [NBP2-33420]

Immunocytochemistry/ Immunofluorescence: Capicua Antibody [NBP2-33420]

Immunocytochemistry/Immunofluorescence: Capicua Antibody [NBP2-33420] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Staining is shown in green.
Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line A-549
Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human cerebellum shows strong nuclear immunoreactivity in Purkinje cells, as well as positivity in neurons in granular and molecular layers.
Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular stromal cells.
Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Applications for Capicua Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Capicua

CIC, a human homolog of Drosophila capicua, encodes a high mobility group box transcription factor and is fused to a double homeodomain gene DUX4 as a result of a recurrent chromosomal translocation. It is involved in epidermal growth factor receptor (EGFR) signaling, developmental patterning, and cell fate. In humans, CIC may be involved in granule cell lineage, cerebellar development, and medulloblastoma tumorigenesis. Recent evidence indicates that CIC is involved in Spinocerebellar Ataxia Type 1 (SCA-1) where it interacts directly with the product of the SCA-1 gene Ataxin-1.

Alternate Names

capicua homolog (Drosophila), KIAA0306capicua (Drosophila) homolog, protein capicua homolog

Gene Symbol

CIC

UniProt

Q96RK0 (Human)

Additional Capicua Products

Product Documents for Capicua Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Capicua Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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