Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88367

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Carbohydrate Sulfotransferase 15/CHST15 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVER

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23457544)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367]

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367]

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367] - Staining of human heart muscle shows strong positivity in myocytes.
Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free [NBP1-88367]

Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free [NBP1-88367]

Staining of human cell line A-431 shows localization to cytosol & centrosome.

Applications for Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbohydrate Sulfotransferase 15/CHST15

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxylgroup of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containingGlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence,GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found inthrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated earlyactivation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAGexpression; however such results are unclear in vivo

Alternate Names

BRAG, CHST15, GalNAc4S-6ST

Gene Symbol

CHST15

Additional Carbohydrate Sulfotransferase 15/CHST15 Products

Product Documents for Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Carbohydrate Sulfotransferase 15/CHST15 Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail