Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

Novus Biologicals | Catalog # NBP3-18130

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-220 of E.coli Carbonic Anhydrase II/CA2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

SDS-PAGE: Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein [NBP3-18130]

SDS-PAGE: Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein [NBP3-18130]

SDS-Page: Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein [NBP3-18130] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP3-18130
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase II/CA2

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes the reversible hydration of carbon dioxide (1). It plays a role in osteoclast resorptive activity by acidification. The acid secretion process is initiated by CA2, which favors carbon dioxide hydration and converts it to bicarbonate. CA2 is also involved in proximal renal tubular bicarbonate reclamation and distal renal tubular acidification (2). Defects in CA2 are associated with osteopetrosis and renal tubular acidosis (1).

Alternate Names

CA2

Gene Symbol

CA2

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

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