Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99972

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Carbonic Anhydrase VB/CA5B Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VB/CA5B (Uniprot#: Q9Y2D0; Cys34-Pro317)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free

Western Blot: Carbonic Anhydrase VB/CA5B Antibody [NBP2-99972]

Western Blot: Carbonic Anhydrase VB/CA5B Antibody [NBP2-99972]

Western Blot: Carbonic Anhydrase VB/CA5B Antibody [NBP2-99972] - Anti-Carbonic Anhydrase VB/CA5B rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse liver tissue Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Carbonic Anhydrase VB/CA5B Antibody [NBP2-99972]

Immunoprecipitation: Carbonic Anhydrase VB/CA5B Antibody [NBP2-99972]

Immunoprecipitation: Carbonic Anhydrase VB/CA5B Antibody [NBP2-99972] - Carbonic Anhydrase VB/CA5B was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-Carbonic Anhydrase VB/CA5B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Carbonic Anhydrase VB/CA5B rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa

Applications for Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase VB/CA5B

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide.CAs show extensive diversity in their distribution and subcellular localization.They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA VB, also known as carbonate dehydratase VB, is one of two isoforms of CA V. It localizes to the mitochondria and is involved in metabolic processes. CA VB is predominantly expressed in heart, pancreas, lung, placenta, kidney and skeletal muscle. It exhibits highest homology with family member CA VA (the second isoform of CA V); however, unlike CA VA, it is not expressed in the liver, suggesting that it plays a significantly different physiological role.

Alternate Names

CA5B, CAVB

Gene Symbol

CA5B

Additional Carbonic Anhydrase VB/CA5B Products

Product Documents for Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free and earn rewards!

Have you used Carbonic Anhydrase VB/CA5B Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies