Carboxypeptidase E/CPE Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15699

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (94%), Chimpanzee (99%), Porcine (95%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Carboxypeptidase E/CPE Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase E/CPE. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (83%). Rat reactivity reported from a verified customer review.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carboxypeptidase E/CPE Antibody - BSA Free

Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections. Image from verified customer review.
Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Immunohistochemistry-Paraffin: Carboxypeptidase E/CPE Antibody [NBP2-15699]

Immunohistochemistry-Paraffin: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Human breast cancer. Carboxypeptidase E antibody [N2C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for Carboxypeptidase E/CPE Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 28978054). Immunohistochemistry-Frozen from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP2-15699 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carboxypeptidase E/CPE

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq]

Alternate Names

CPE, CPH-1

Gene Symbol

CPE

UniProt

P16870

Additional Carboxypeptidase E/CPE Products

Product Documents for Carboxypeptidase E/CPE Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Carboxypeptidase E/CPE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Metalloproteases and Regulators
Obesity

Citations for Carboxypeptidase E/CPE Antibody - BSA Free

Customer Reviews for Carboxypeptidase E/CPE Antibody - BSA Free (1)

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Customer Images

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  • Carboxypeptidase E/CPE Antibody
    Name: Parisa Shabani
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult heart
    Species: Rat
    Verified Customer | Posted 06/27/2022
    Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections
    Carboxypeptidase E/CPE Antibody - BSA Free NBP2-15699
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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