Carm1 [p Ser228] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16892

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Carm1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S228 of human PRMT4/CARM1 (NP_954592.1). VKSNN

Modification

p Ser 228

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carm1 [p Ser228] Antibody - Azide and BSA Free

Western Blot: Carm1 [p Ser228] AntibodyAzide and BSA Free [NBP3-16892]

Western Blot: Carm1 [p Ser228] AntibodyAzide and BSA Free [NBP3-16892]

Western Blot: Carm1 [p Ser228] Antibody [NBP3-16892] - Western blot analysis of extracts of Jurkat cells, using Carm1 antibody (NBP3-16892) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Carm1 [p Ser228] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Carm1

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 (PubMed 11724789)).(supplied by OMIM)

Long Name

Coactivator-associated Arginine Methyltransferase 1

Alternate Names

PRMT4

Gene Symbol

CARM1

Additional Carm1 Products

Product Documents for Carm1 [p Ser228] Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Carm1 [p Ser228] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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