Casein Kinase 2 alpha Antibody (S05-7F8)

Western Blot: Casein Kinase 2 alpha Antibody (S05-7F8) [NBP3-19853]

Western Blot: Casein Kinase 2 alpha Antibody (S05-7F8) [NBP3-19853] - Western blot detection of Casein Kinase 2 alpha in Hela (Lane 1), CHO-K1 (Lane 2), C6 (Lane 3), mouse Brain (Lane 4), Ramos (Lane 5) using NBP3-19853 (1:1000 diluted)

Western Blot Shows Human Casein Kinase 2 alpha Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and Casein Kinase 2 alpha knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with Casein Kinase 2 alpha Antibody (Catalog # NBP3-19853) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for Casein Kinase 2 alpha (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Knockout Validation of Casein Kinase 2 alpha Antibody in HAP1 and Casein Kinase 2 alpha Knockout HAP1 Cells in Immunocytochemistry/Immunofluorescence.

HAP1 WT and Casein Kinase 2 alpha KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Casein Kinase 2 alpha antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/300. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).