Caspase-1 Antibody Pair [Biotin]

Sandwich ELISA

0.15ng/ml - 10 ng/ml

Cell Culture Supernatant (100 ul)

50 pg/ml

Mouse

Sandwich ELISA for the quantitative determination of Mouse Caspase-1

100 ul

Biotin

1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (mouse monoclonal antibody) to 2 ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (0.5 mg/ml) (biotinylated mouse monoclonal antibody) to 1 ug/ml in ELISA Buffer and use it fresh.
3. Reconstitute the Streptavidin-HRP (STREP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
4. Reconstitute the Standard Protein (STD) (recombinant mouse Caspase-1) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 10 ng/ml, 5 ng/ml, 2.5 ng/ml, 1.25 ng/ml, 0.625 ng/ml, 0.3125 ng/ml, 0.15625 ng/ml and 0 ng/ml.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.