Human Caspase-8 ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 6.7%

Inter-Assay Precision (Precision between assays) CV% 8.5%

Recovery for Human Caspase-8 ELISA Kit (Colorimetric)

Scientific Data Images for Human Caspase-8 ELISA Kit (Colorimetric)

Kit Contents for Human Caspase-8 ELISA Kit (Colorimetric)

• Adhesive Films
• Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
• anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
• Anti-rabbit-IgG-HRP
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Human Caspase-8 Standard lyophilized, 20.00 ng/ml upon reconstitution
• Lysis Buffer 10x
• Sample Diluent
• Stop Solution (1M Phosphoric acid)
• Substrate Solution (tetramethyl-benzidine)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Background: Caspase-8

Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-8 has been reported to have additional functions unrelated to cell death. It is required for T-cell homeostasis, proliferation, and cell activation.

Additional Caspase-8 Products

• All Products for Caspase-8
• Caspase-8 Activity Assays
• Caspase-8 ELISA Kits
• Caspase-8 Lysates
• Caspase-8 Primary Antibodies
• Caspase-8 Proteins and Enzymes
• Caspase-8 Small Molecules and Peptides

Product Documents for Human Caspase-8 ELISA Kit (Colorimetric)